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VOL. 11, ISSUE 2 (2026)
In vitro seed germination and phytochemical screening of Dendrobium Crepidatum Lindl. & Paxton, a medicinal orchid species of Bangladesh
Authors
Tripa Paul, Bishakha Chowdhury, M d Mahbubur Rahman, Tapash Kumar Bhowmik
Abstract
Dendrobium crepidatum Lindl. & Paxton is a
medicinally significant, epiphytic orchid native to Bangladesh, currently
facing severe population decline due to habitat destruction and unregulated
over collection. This study aimed to establish a reliable protocol for in
vitro mass propagation and to comparatively evaluate the phytochemical
profiles of naturally grown versus in vitro derived plantlets.
Asymbiotic seed germination was tested on four basal media (KC, MS, MVW and
PM), with Phytamax (PM) medium yielding the highest germination frequency
(58.34%). After seedlings development successful acclimatization were completed
in outside environment with a 80% survival rate. Phytochemical screening
evaluated secondary metabolites, confirming the abundant presence of alkaloids,
flavonoids, phenols and terpenoids across various tissues. In vitro
derived plantlets displayed a robust secondary metabolite profile remarkably
comparable to wild ecotypes. In vitro plantlets exhibited phytochemical
profiling and validating plant tissue culture as a sustainable alternative for
the commercial propagation and ex situ conservation of this near
threatened therapeutic orchid species.
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Pages:42-45
How to cite this article:
Tripa Paul, Bishakha Chowdhury, M d Mahbubur Rahman, Tapash Kumar Bhowmik "<i>In vitro</i> seed germination and phytochemical screening of <i>Dendrobium Crepidatum</i> Lindl. & Paxton, a medicinal orchid species of Bangladesh". International Journal of Academic Research and Development, Vol 11, Issue 2, 2026, Pages 42-45
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